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hASCs' relative <t>metabolic</t> <t>activity</t> in hPLMA and Matrigel hydrogels for five time points, assessed through the CCK-8 assay. Results are expressed as mean (SD) with n = 5 ( A. ) Representative fluorescence images of hASCs encapsulated in hPLMA and Matrigel hydrogels. The DAPI/Ki-67 staining was performed at 0, 3, and 7 days of culture, with Ki-67 expression (red) and DAPI staining <t>cells'</t> nucleus (blue). The white arrow points to an example of Ki-67 stained cells. Scale bar: 200 μm. ( B. ) Fold change in Ki-67 expression calculated from the CTCF equation using the fluorescence images of Ki-67 expression cultured for 3 and 7 days. The fold change is here compared to day 0. ( C. ) Results are expressed as mean (SD) with n = 3. ∗ p-value <0.05, ∗∗ p-value <0.01, ∗∗∗ p-value <0.001 and ∗∗∗∗ p-value <0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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hASCs' relative <t>metabolic</t> <t>activity</t> in hPLMA and Matrigel hydrogels for five time points, assessed through the CCK-8 assay. Results are expressed as mean (SD) with n = 5 ( A. ) Representative fluorescence images of hASCs encapsulated in hPLMA and Matrigel hydrogels. The DAPI/Ki-67 staining was performed at 0, 3, and 7 days of culture, with Ki-67 expression (red) and DAPI staining <t>cells'</t> nucleus (blue). The white arrow points to an example of Ki-67 stained cells. Scale bar: 200 μm. ( B. ) Fold change in Ki-67 expression calculated from the CTCF equation using the fluorescence images of Ki-67 expression cultured for 3 and 7 days. The fold change is here compared to day 0. ( C. ) Results are expressed as mean (SD) with n = 3. ∗ p-value <0.05, ∗∗ p-value <0.01, ∗∗∗ p-value <0.001 and ∗∗∗∗ p-value <0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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hASCs' relative <t>metabolic</t> <t>activity</t> in hPLMA and Matrigel hydrogels for five time points, assessed through the CCK-8 assay. Results are expressed as mean (SD) with n = 5 ( A. ) Representative fluorescence images of hASCs encapsulated in hPLMA and Matrigel hydrogels. The DAPI/Ki-67 staining was performed at 0, 3, and 7 days of culture, with Ki-67 expression (red) and DAPI staining <t>cells'</t> nucleus (blue). The white arrow points to an example of Ki-67 stained cells. Scale bar: 200 μm. ( B. ) Fold change in Ki-67 expression calculated from the CTCF equation using the fluorescence images of Ki-67 expression cultured for 3 and 7 days. The fold change is here compared to day 0. ( C. ) Results are expressed as mean (SD) with n = 3. ∗ p-value <0.05, ∗∗ p-value <0.01, ∗∗∗ p-value <0.001 and ∗∗∗∗ p-value <0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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hASCs' relative <t>metabolic</t> <t>activity</t> in hPLMA and Matrigel hydrogels for five time points, assessed through the CCK-8 assay. Results are expressed as mean (SD) with n = 5 ( A. ) Representative fluorescence images of hASCs encapsulated in hPLMA and Matrigel hydrogels. The DAPI/Ki-67 staining was performed at 0, 3, and 7 days of culture, with Ki-67 expression (red) and DAPI staining <t>cells'</t> nucleus (blue). The white arrow points to an example of Ki-67 stained cells. Scale bar: 200 μm. ( B. ) Fold change in Ki-67 expression calculated from the CTCF equation using the fluorescence images of Ki-67 expression cultured for 3 and 7 days. The fold change is here compared to day 0. ( C. ) Results are expressed as mean (SD) with n = 3. ∗ p-value <0.05, ∗∗ p-value <0.01, ∗∗∗ p-value <0.001 and ∗∗∗∗ p-value <0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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hASCs' relative metabolic activity in hPLMA and Matrigel hydrogels for five time points, assessed through the CCK-8 assay. Results are expressed as mean (SD) with n = 5 ( A. ) Representative fluorescence images of hASCs encapsulated in hPLMA and Matrigel hydrogels. The DAPI/Ki-67 staining was performed at 0, 3, and 7 days of culture, with Ki-67 expression (red) and DAPI staining cells' nucleus (blue). The white arrow points to an example of Ki-67 stained cells. Scale bar: 200 μm. ( B. ) Fold change in Ki-67 expression calculated from the CTCF equation using the fluorescence images of Ki-67 expression cultured for 3 and 7 days. The fold change is here compared to day 0. ( C. ) Results are expressed as mean (SD) with n = 3. ∗ p-value <0.05, ∗∗ p-value <0.01, ∗∗∗ p-value <0.001 and ∗∗∗∗ p-value <0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Journal: Materials Today Bio

Article Title: Redefining 3D cell culture: Human methacryloyl platelet lysates hydrogels for reliable, consistent, and ethical applications

doi: 10.1016/j.mtbio.2026.103443

Figure Lengend Snippet: hASCs' relative metabolic activity in hPLMA and Matrigel hydrogels for five time points, assessed through the CCK-8 assay. Results are expressed as mean (SD) with n = 5 ( A. ) Representative fluorescence images of hASCs encapsulated in hPLMA and Matrigel hydrogels. The DAPI/Ki-67 staining was performed at 0, 3, and 7 days of culture, with Ki-67 expression (red) and DAPI staining cells' nucleus (blue). The white arrow points to an example of Ki-67 stained cells. Scale bar: 200 μm. ( B. ) Fold change in Ki-67 expression calculated from the CTCF equation using the fluorescence images of Ki-67 expression cultured for 3 and 7 days. The fold change is here compared to day 0. ( C. ) Results are expressed as mean (SD) with n = 3. ∗ p-value <0.05, ∗∗ p-value <0.01, ∗∗∗ p-value <0.001 and ∗∗∗∗ p-value <0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Biological Characterization – Cell Metabolic Activity Quantification: The Cell Counting Kit-8 assay (CCK-8, MCE®, USA) was applied to quantify hASCs' metabolic activity.

Techniques: Activity Assay, CCK-8 Assay, Fluorescence, Staining, Expressing, Cell Culture